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KMID : 0613820100200030326
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Journal of Life Science
2010 Volume.20 No. 3 p.326 ~ p.335
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Radical Scavenging Activities of Phellinus pini
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Nam Byung-Hyouk
Jo Wol-Soon
Cui-Yong
Choi Yoo-Jin
Lee Jae-Dong
Jeong Min-Ho
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Abstract
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The concentration of phenolics in Phellinus pini (CY001) extracts, expressed as mg of GAEs per g of P. pini fractions, and the EtOAc fraction (436.5 ㎎ GAEs/g) of P. pini had a higher phenolic content than other fractions. Several biochemical assays were used to screen antioxidant properties such as reducing power, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, NBT/XO superoxide system and inhibition of DCF/AAPH peroxyl radicals. Among the six mushroom extracts, the EtOAc fraction from P. pini (CY001) showed the most potent DPPH radical, superoxide radical, and peroxyl radical scavenging activities, with IC50 values of 11.49 ㎍/ml, 8.32 ㎍/㎖, and 1.91 μg/㎖, respectively. The EtOAc fraction of P. pini (CY001) significantly inhibited enzymatic lipid peroxidation and effectively attenuated LPS-induced NO production of RAW 264.7 cells without cytotoxicity. We also found that the EtOAc fraction had a significant hepato-protectant effect on tacrine-induced cytotoxicity in HepG2 cells. These findings suggest that P. pini (CY001) may have potential as a natural antioxidant, which contains compound(s) with radical scavenging activity.
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KEYWORD
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Phellinus pini, free radicals, phenol content, nitric oxide, hepatoprotection
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